Comments on: Ping-pong studies of signaling pathways in hematopoietic stem cells http://hematopoiesis.info/2009/07/22/ping-pong-studies-of-signaling-pathways-in-hematopoietic-stem-cells/ Blood Stem Cell & Cell Therapy Trends Mon, 14 May 2012 16:25:58 +0000 hourly 1 http://wordpress.org/?v=3.3.2 By: Mr. Gunn http://hematopoiesis.info/2009/07/22/ping-pong-studies-of-signaling-pathways-in-hematopoietic-stem-cells/#comment-11557 Mr. Gunn Wed, 05 Aug 2009 23:33:46 +0000 http://hematopoiesis.info/?p=713#comment-11557 We had a similar situation with Wnt in MSCs. One group (DeBoer) used Li ions to inhibit GSK3beta, saw increasd proliferation of cells and determined that, as in many cancers, Wnt promoted cellular proliferation. Our group came along and showed that Dkk1, a Wnt antagonist, promoted proliferation and also inhibited differentiation. However, we also found that Li ions inhibited differentiation. We couldn't resolve this until we found a more specific inhibitor of GSK3beta, which supported our results. Now you can find papers about the "biphasic" effect of lithium, depending on dose. Certainly, pong-pong results happen all the time. This is just an indicator of possible outcomes and illustrates how you should set up your experiments to consider both of the ping-pong possibilities, not just the evidence for one side, regardless of how strong it is, on its own. We had a similar situation with Wnt in MSCs. One group (DeBoer) used Li ions to inhibit GSK3beta, saw increasd proliferation of cells and determined that, as in many cancers, Wnt promoted cellular proliferation.

Our group came along and showed that Dkk1, a Wnt antagonist, promoted proliferation and also inhibited differentiation. However, we also found that Li ions inhibited differentiation. We couldn’t resolve this until we found a more specific inhibitor of GSK3beta, which supported our results. Now you can find papers about the “biphasic” effect of lithium, depending on dose.

Certainly, pong-pong results happen all the time. This is just an indicator of possible outcomes and illustrates how you should set up your experiments to consider both of the ping-pong possibilities, not just the evidence for one side, regardless of how strong it is, on its own.

]]> By: Toni http://hematopoiesis.info/2009/07/22/ping-pong-studies-of-signaling-pathways-in-hematopoietic-stem-cells/#comment-11553 Toni Wed, 05 Aug 2009 10:16:38 +0000 http://hematopoiesis.info/?p=713#comment-11553 vav-Cre more specifically for hematopoietic cells Mx1-Cre a little bit generally in bone marrow tissue, like MSC. It might be related with the contrast results. vav-Cre more specifically for hematopoietic cells
Mx1-Cre a little bit generally in bone marrow tissue, like MSC.

It might be related with the contrast results.

]]>