This picture shows one of the reasons why I was stuck in the lab for a couple of nights.
It’s a mouse liver and spleen – and together, they took up almost the whole abdominal cavity! The spleen was 12 times bigger than a normal one. You can see that the normal structure of liver was basically replaced by a lot of “tumor-like” nodes. So it seems that this animal died from cancer or metastasis.
But the magic is that I didn’t even inject any tumor cells or insert any oncogenes to cause these nodes, nor were they caused by parasites.
It happened when I just serially transplanted bone marrow cells in the 4th recipient from one of my genetically modified mice.
Flow cytometry showed me that these white infiltrates are the donor’s myeloid cells, and moreover – they are immature, because a lot of them expressed the stem cell marker c-Kit. So this is a leukemic (or MPD) death!